Electron Microscopy Data Bank

EMD-20566

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The axonemal doublet microtubule focusing on the I1 dynein extracted from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas wild type cilia

Release date: Aug. 12, 2020
Resolution: 38.0 Å
EM Method: Subtomogram averaging

Fu G, Scarbrough C, Song K, Phan N, Wirschell M, Nicastro D

FASEB J (2021) 35 pp. e21646-e21646 [ Pubmed: 33993568 DOI: doi:10.1096/fj.202001857R ]

Sample:

The i1 dynein averaged from chlamydomonas wild type cilia (Cellular component from Chlamydomonas reinhardtii)

EMD-20567

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The axonemal doublet microtubule focusing on the I1 dynein region extracted from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas ida7-1 mutant cilia

Release date: Aug. 12, 2020
Resolution: 40.0 Å
EM Method: Subtomogram averaging

Fu G, Scarbrough C, Song K, Phan N, Wirschell M, Nicastro D

FASEB J (2021) 35 pp. e21646-e21646 [ Pubmed: 33993568 DOI: doi:10.1096/fj.202001857R ]

Sample:

The i1 dynein averaged from chlamydomonas ida7-1 mutant cilia (Cellular component from Chlamydomonas reinhardtii)

EMD-20568

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The axonemal doublet microtubule focusing on the I1 dynein region extracted from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas tctex2b mutant cilia

Release date: Aug. 12, 2020
Resolution: 39.0 Å
EM Method: Subtomogram averaging

Fu G, Scarbrough C, Song K, Phan N, Wirschell M, Nicastro D

FASEB J (2021) 35 pp. e21646-e21646 [ Pubmed: 33993568 DOI: doi:10.1096/fj.202001857R ]

Sample:

The i1 dynein averaged from chlamydomonas tctex2b cilia (Cellular component from Chlamydomonas reinhardtii)

EMD-7805

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Subtomogram average (699 axonemal repeats) of isolated Rib72A/B double knock out Tetrahymena cilia showing the lumen of the ciliary doublet microtubule (DMT)

Release date: Sept. 5, 2018
Resolution: 40.1 Å
EM Method: Subtomogram averaging

Stoddard D, Zhao Y, Bayless BA, Gui L, Louka P, Dave D, Suryawanshi S, Tomasi RF, Dupuis-Williams P, Baroud CN, Gaertig J, Winey M, Nicastro D

Mol Biol Cell (2018) 29 pp. 2566-2577 [ Pubmed: 30133348 DOI: doi:10.1091/mbc.E18-06-0405 ]

Sample:

Subtomogram average (699 axonemal repeats) of isolated rib72a/b double knock out tetrahymena cilia showing the lumen of the ciliary doublet microtubule (dmt) (Cellular component from Tetrahymena thermophila)

EMD-7806

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Subtomogram average (758 axonemal repeats) of isolated Rib72A knock out Tetrahymena cilia showing the lumen of the ciliary doublet microtubule (DMT)

Release date: Sept. 5, 2018
Resolution: 40.1 Å
EM Method: Subtomogram averaging

Stoddard D, Zhao Y, Bayless BA, Gui L, Louka P, Dave D, Suryawanshi S, Tomasi RF, Dupuis-Williams P, Baroud CN, Gaertig J, Winey M, Nicastro D

Mol Biol Cell (2018) 29 pp. 2566-2577 [ Pubmed: 30133348 DOI: doi:10.1091/mbc.E18-06-0405 ]

Sample:

Subtomogram average (758 axonemal repeats) of isolated rib72a knock out tetrahymena cilia showing the lumen of the ciliary doublet microtubule (dmt) (Cellular component from Tetrahymena thermophila)

EMD-7807

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Subtomogram average (735 axonemal repeats) of isolated Rib72B knock out Tetrahymena cilia Rescued with Rib72B-GFP, showing the lumen of the ciliary doublet microtubule (DMT)

Release date: Sept. 5, 2018
Resolution: 40.1 Å
EM Method: Subtomogram averaging

Stoddard D, Zhao Y, Bayless BA, Gui L, Louka P, Dave D, Suryawanshi S, Tomasi RF, Dupuis-Williams P, Baroud CN, Gaertig J, Winey M, Nicastro D

Mol Biol Cell (2018) 29 pp. 2566-2577 [ Pubmed: 30133348 DOI: doi:10.1091/mbc.E18-06-0405 ]

Sample:

Subtomogram average (735 axonemal repeats) of isolated rib72b knock out tetrahymena cilia rescued with rib72b-gfp, showing the lumen of the ciliary doublet microtubule (dmt) (Cellular component from Tetrahymena thermophila)

EMD-7811

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Subtomogram average (702 axonemal repeats) of isolated Rib72B knock out Tetrahymena cilia showing the lumen of the ciliary doublet microtubule (DMT)

Release date: Sept. 5, 2018
Resolution: 40.1 Å
EM Method: Subtomogram averaging

Stoddard D, Zhao Y, Bayless BA, Gui L, Louka P, Dave D, Suryawanshi S, Tomasi RF, Dupuis-Williams P, Baroud CN, Gaertig J, Winey M, Nicastro D

Mol Biol Cell (2018) 29 pp. 2566-2577 [ Pubmed: 30133348 DOI: doi:10.1091/mbc.E18-06-0405 ]

Sample:

Subtomogram average (702 axonemal repeats) of isolated rib72b knock out tetrahymena cilia showing the lumen of the ciliary doublet microtubule (dmt) (Cellular component from Tetrahymena thermophila)

EMD-2912

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Cryo-electron tomograms of EB1 conjugated to gold nanoparticles, in interaction with growing microtubule ends

Release date: Sept. 21, 2016
EM Method: Tomography

Guesdon A, Bazile F, Buey RM, Mohan R, Monier S, Angevin M, Heichette C, Tampe R, Duchesne L, Akhmanova A, Steinmetz MO, Chretien D

Nat Cell Biol (2016) 18 pp. 1102-1108 [ Pubmed: 27617931 DOI: doi:10.1038/ncb3412 ]

Sample:

Eb1 conjugated to 6.5 nm gold nanoparticles, in interaction with a microtubule growing end (Sample)
Tubulin alpha chain (500 kDa, Protein from Sus scrofa)
Tubulin beta chain (498 kDa, Protein from Sus scrofa)
End-binding protein one (30 kDa, Protein from Homo sapiens)

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EMD-2916

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Cryo-electron tomograms of EB1 conjugated to gold nanoparticles, in interaction with growing microtubule ends

Release date: Sept. 21, 2016
EM Method: Tomography

Guesdon A, Bazile F, Buey RM, Mohan R, Monier S, Angevin M, Heichette C, Tampe R, Duchesne L, Akhmanova A, Steinmetz MO, Chretien D

Nat Cell Biol (2016) 18 pp. 1102-1108 [ Pubmed: 27617931 DOI: doi:10.1038/ncb3412 ]

Sample:

Tubulin beta chain (498 kDa, Protein from Sus scrofa)
Tubulin alpha chain (500 kDa, Protein from Sus scrofa)
Eb1 conjugated to 9.8 nm gold nanoparticles, in interaction with a microtubule growing end (Sample)
End-binding protein one (30 kDa, Protein from Homo sapiens)

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EMD-2918

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Outwardly curved tubulin sheet at the tip of a growing microtubule

Release date: Sept. 21, 2016
EM Method: Tomography

Guesdon A, Bazile F, Buey RM, Mohan R, Monier S, Angevin M, Heichette C, Tampe R, Duchesne L, Akhmanova A, Steinmetz MO, Chretien D

Nat Cell Biol (2016) 18 pp. 1102-1108 [ Pubmed: 27617931 DOI: doi:10.1038/ncb3412 ]

Sample:

Tubulin beta chain (498 kDa, Protein from Sus scrofa)
Tubulin alpha chain (500 kDa, Protein from Sus scrofa)
End-binding protein one (30 kDa, Protein from Homo sapiens)
Eb1 conjugated to 9.8 nm gold nanoparticles, in interaction with a microtubule growing end. (Sample)

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